Affordable test to determine HIV Infection: Human immunodeficiency virus (HIV 1/O/2), the etiologic agent of the acquired immunodeficiency syndrome (AIDS), is a cytopathic retrovirus. This test uses recombinant antigen sources and detects antibodies by specific immune binding and subsequent chemiluminescent reaction (ICMA technology). Sensitivity and specificity of this assay are 100% and 99.9%, respectively. Sera that are repeatedly reactive in two out of three tests will reflex to HIV-1/HIV-2 supplemental testing. Some individuals may be initially reactive by the preliminary test and negative or indeterminate by HIV-1/HIV-2 supplemental testing. This may be caused by other viral antibodies or autoantibodies that cross-react with the viral antigens, although this is extremely rare.